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American Cleanroom Systems is a full-service manufacturer, specializing in the rapid production and installation of superior quality custom Pharmaceutical, Medical and Industrial cleanrooms.
Our team can design, manufacture and install your certified cleanroom in as little as 4 weeks,
on-site with minimum disruption.
View FAQs about Cleanroom Classifications
US FED STD 209E Cleanroom Standards | Cleanroom Classifications
Class | Maximum Particles/ft³ | ISO equivalent | ||||
>0.1 um | >0.2 um | >0.3 um | >0.5 um | >5 um | ||
1 | 35 | 7 | 3 | 1 | ISO3 | |
10 | 350 | 75 | 30 | 10 | ISO4 | |
100 | 100 | ISO5 | ||||
1000 | 1000 | 7 | ISO6 | |||
10,000 | 10,000 | 70 | ISO7 | |||
100,000 | 100,000 | 700 | ISO8 |
ISO 14644-1 Cleanroom Standards | Cleanroom Classifications
Class | Maximum Particles/m³ | FED STD 209E equivalent | |||||
>0.1 um | >0.2 um | >0.3 um | >0.5 um | >1 um | >5 um | ||
ISO 1 | 10 | 2 | |||||
ISO 2 | 100 | 24 | 10 | 4 | |||
ISO 3 | 1,000 | 237 | 102 | 35 | 8 | Class 1 | |
ISO 4 | 10,000 | 2,370 | 1,020 | 352 | 83 | Class 10 | |
ISO 5 | 100,000 | 23,700 | 10,200 | 3,520 | 832 | 29 | Class 100 |
ISO 6 | 1,000,000 | 237,000 | 102,000 | 35,200 | 8,320 | 293 | Class 1,000 |
ISO 7 | 352,000 | 83,200 | 2,930 | Class 10,000 | |||
ISO 8 | 3,520,000 | 832,000 | 29,300 | Class 100,000 | |||
ISO 9 | 35,200,000 | 8,320,000 | 293,000 | Room Air |
Design Requirements for Cleanroom Classifications
Criteria | Class 10 ISO4 | Class 100 ISO5 | Class 1000 ISO6 | Class 10,000 ISO7 | Class 100,000 ISO8 |
Air changes per HR/Min | 500-600 / 8 to 10 | 300 to 480 / 5 to 8 | 180 / 3 | 60 /1 | 20 /0.33 |
Filter coverage % | 90 – 100 | 60 – 70 | 20 – 30 | 7 – 15 | 4 – 5 |
CFM per square foot | 85 – 90 | 36 – 65 | 18 – 32 | 9 – 16 | 4 – 8 |
Filter Efficiency | 99.9997% ULPAs | 99.997% HEPAs | 99.997% HEPAs | 99.997% HEPAs | 99.97% HEPAs |
Ceiling Type | Aluminum T-bar grid | Aluminum T-bar grid | Aluminum T-bar grid | Conventional T-bar grid | Conventional T-bar grid |
Light Fixture type | Tear drop or Flow thru | Tear drop or 2’x4’ cleanroom fixture | 2’x4’ cleanroom fixture | 2’x4’ cleanroom fixture | 2’x4’ standard fixture |
Ceiling Panel | FRP, Vinyl rock or Mylar | FRP, Vinyl rock or Mylar | Vinyl rock or Mylar | Vinyl rock or Mylar | Vinyl rock or Mylar |
Wall System | Modular or standard built | Modular or standard built | Modular or standard built | Modular or drywall | Modular or drywall |
Flooring cover | Welded sheet vinyl or Epoxy | Welded sheet vinyl or Epoxy | Welded Sheet vinyl or Epoxy | Sheet vinyl or VCT | Sheet vinyl or VCT |
Flooring base | 2” to 6” cove | Cove or Aluminum base channel | Cove or Aluminum base channel | Cove or Aluminum base channel | Cove or Aluminum base channel |
Air Returns | Raised floor or center returns | Low wall on long axis | Low wall at perimeter | Low wall | Low wall or ceiling |
GMP Annex European Community Cleanroom Standards
Grade | At Rest >0.5 um/M3 | At Rest >5um/M3 | Operational >0.5um/M3 | Operational >0.5um/M3 | Equivalent ISO at rest/operational | Equivalent Fed209E at rest / operational |
---|---|---|---|---|---|---|
A | 3,520 | 20 | 3,520 | 20 | ISO-5/5 | 100/100 |
B | 3,520 | 29 | 352,000 | 2,900 | ISO-5/7 | 100/10k |
C | 352,000 | 2,900 | 3,520,000 | 29,000 | ISO-7/8 | 10k/100k |
D | 3,520,000 | 29,000 | not defined | not defined | ISO-8/na | 100k/na |
![Clean Room Classifications & ISO Standards (5) Clean Room Classifications & ISO Standards (5)](https://i0.wp.com/www.americancleanrooms.com/wp-content/uploads/2022/04/Cleanroom-Airflow-Recirculating-Modular-Cleanroom-2022.gif)
Types of Cleanrooms:
![Clean Room Classifications & ISO Standards (6) Clean Room Classifications & ISO Standards (6)](https://i0.wp.com/www.americancleanrooms.com/wp-content/uploads/2022/04/Modular-Cleanroom-500x438.jpg)
Modular Cleanroom
A Modular Cleanroom is a cleanroom built using prefabricated modular components then assembled on-site. Why choose modular cleanrooms?
- Modular cleanrooms can be manufactured and installed 50% faster than stick built conventional construction cleanrooms.
- Unlike conventional construction cleanrooms, modular cleanrooms are easily modified, expanded, or relocated.
- Modular cleanrooms can be built for any classification (class 100-100k, ISO5-ISO8) with temperature and RH control.
![Clean Room Classifications & ISO Standards (7) Clean Room Classifications & ISO Standards (7)](https://i0.wp.com/www.americancleanrooms.com/wp-content/uploads/2022/04/Hybrid-Cleanroom-500x438.jpg)
A Hybrid Cleanroom is a cleanroom built combining modular components with existing conventional construction walls. When to use a hybrid cleanroom design?
- Combine modular cleanroom air returns and internal walls with existing conventional construction walls can offer cost savings.
- The use of modular cleanroom components significantly speeds up installation of cleanroom.
- Hybrid works best for less demanding ISO-7 or ISO-8 class cleanrooms.
![Clean Room Classifications & ISO Standards (8) Clean Room Classifications & ISO Standards (8)](https://i0.wp.com/www.americancleanrooms.com/wp-content/uploads/2022/04/Softwall-Cleanroom-500x438.jpg)
Softwall Cleanroom
A Softwall Cleanroom is a cleanroom created by hanging clear vinyl curtains on an aluminum frame to create walls.
- Softwall cleanrooms are one pass. Filtered air is pulled by HEPA fan filter units into the cleanroom. The air passes under the curtain back into the surrounding room.
- Softwall cleanrooms can be mounted on casters so they can be easily relocated.
- Unlike recirculating modular cleanrooms, you cannot add air conditioning to softwall cleanrooms.
![Clean Room Classifications & ISO Standards (9) Clean Room Classifications & ISO Standards (9)](https://i0.wp.com/www.americancleanrooms.com/wp-content/uploads/2022/04/Medical-Device-Cleanroom-500x438.jpg)
Medical Device Cleanroom
A Medical Device Cleanroom is a cleanroom that is used to manufacture medical devices.
- Medical device cleanrooms are designed to provide a controlled environment as specified by the device approved FDA validation and CGMP practice.
- The modular cleanroom is optimized to create a sterile manufacturing environment for the medical devices.
- Often FRP modular cleanroom walls are chosen for medical device cleanrooms because of the frequent cleaning done in these types of cleanrooms.
Pharmaceutical Cleanroom
A Pharmaceutical Cleanroom is a cleanroom that is used for pharmaceutical manufacturing.
- Pharmaceutical cleanrooms are designed to provide a controlled environment as specified by your approved FDA validation and CGMP practice.
- The modular cleanroom is optimized to create a sterile manufacturing environment for the pharmaceutical products.
- Filling rooms are typically ISO5 class 100.
- FRP modular cleanroom walls are standard due to the aggressive chemicals used to clean pharmaceutical cleanrooms.
![Clean Room Classifications & ISO Standards (11) Clean Room Classifications & ISO Standards (11)](https://i0.wp.com/www.americancleanrooms.com/wp-content/uploads/2022/04/Turnkey-Complete-Cleanroom-500x438.jpg)
Turnkey Complete Cleanroom
A Turnkey Complete Cleanroom is when the modular cleanroom company provides beginning to end service, doing all aspects of the cleanroom project. This includes:
- Modular cleanroom design
- Manufacturing of modular cleanroom material
- Installation of modular cleanroom
- HVAC, electrical, and flooring provided
- Certification of modular cleanroom
![Clean Room Classifications & ISO Standards (12) Clean Room Classifications & ISO Standards (12)](https://i0.wp.com/www.americancleanrooms.com/wp-content/uploads/2022/04/Mask-Manufacturing-Cleanroom-500x438.jpg)
Mask Manufacturing Cleanroom
A Mask Manufacturing Cleanroom is a cleanroom that is used for manufacturing of K95 and surgical masks during the Covid-19 crisis.
- The cleanroom is designed to provide a sterile controlled environment due to mask classification as medical device
- Special modular cleanroom design for rapid manufacturing in factory and installation by customer in field
- Using modular cleanroom walls systems allowed existing warehouse to be quickly converted into cleanroom at reasonable price
![Clean Room Classifications & ISO Standards (13) Clean Room Classifications & ISO Standards (13)](https://i0.wp.com/www.americancleanrooms.com/wp-content/uploads/2022/04/Laser-Cleanroom-500x438.jpg)
Laser Cleanroom
A Laser Cleanroom is a cleanroom optimized for sensitive laser experiments.
- Cleanroom blackout curtains
- HEPA filtration to remove air particulates in cleanroom that could influence experiments
- Tight temperature and humidity control for the cleanroom space
![Clean Room Classifications & ISO Standards (14) Clean Room Classifications & ISO Standards (14)](https://i0.wp.com/www.americancleanrooms.com/wp-content/uploads/2022/04/Static-Dissipative-Cleanroom-500x438.jpg)
Static Dissipative Cleanroom
A Static Dissipative Cleanroom is designed to prevent static from building up within the cleanroom which can damage sensitive electronic components. Features include:
- Static dissipative modular cleanroom walls
- Static dissipative cleanroom flooring
- Humidity control
- Ionizer bars
![Clean Room Classifications & ISO Standards (15) Clean Room Classifications & ISO Standards (15)](https://i0.wp.com/www.americancleanrooms.com/wp-content/uploads/2022/04/E-Liquid-Cleanroom-500x438.jpg)
E-liquid Cleanroom
An E-liquid Cleanroom is designed for mixing of E-liquids for electronic cigarettes.
- E-liquid cleanrooms provide a controlled clean environment for manufacturing of e-liquid ingredients per government regulations
- Modular cleanroom features result in quick installation and easy expansion of the e-liquid cleanroom
- Modular cleanroom is an economical solution for a smaller size cleanroom used by many e-liquid companies.
![Clean Room Classifications & ISO Standards (16) Clean Room Classifications & ISO Standards (16)](https://i0.wp.com/www.americancleanrooms.com/wp-content/uploads/2022/04/USP797-800-Cleanroom-500x438.jpg)
USP797/800 Cleanrooms
USP797/800 Cleanrooms are used for compounding pharmacies.
- Sterile or negative pressure cleanroom is required depending on type of drug being compounded.
- USP797/800 cleanrooms typically require ISO7 compounding rooms, gloveboxes and ISO8 gown rooms.
- A modular cleanroom is an economical solution for the wide variety of USP797/800 cleanroom sizes.
![Clean Room Classifications & ISO Standards (17) Clean Room Classifications & ISO Standards (17)](https://i0.wp.com/www.americancleanrooms.com/wp-content/uploads/2022/04/CBD-Extraction-Cleanroom-500x438.jpg)
CBD Extraction Cleanroom
A CBD Extraction Cleanroom is required for FDA regulated CGMP practice. Typical features include:
- ISO7 or ISO8 cleanroom with HEPA fan filter units to filter the air in the cleanroom
- Cleanable modular cleanroom walls
- Gowning room airlock to don cleanroom garments
- Magnehelic gauges to measure air pressure of cleanroom vs. ambient.
Common Cleanroom Standards
- Fed209E. US government standard for cleanroom classification issue by the GSA. Measurements are in particles/cubic foot. It was superseded in 2021 by ISO 14644-01 -
- View Source
- ISO 14644-01. ISO standard for cleanroom classification. Measurements are in particles/cubic meter.
- FDA GMP or CGMP: FDA standards for Current Good Manufacturing Practices for pharmaceutical, drug, cosmetics, food, nutraceutical and products regulated by the US Food and Drug Administration
- EU-GMP Annex 1: European standards for drug manufacturing
- USP797 and 800 cleanroom standards hazardous and sterile compounding pharmacies
- NSF ANSI National Sanitary Foundation standards for food drink processing
- BSL1-4 : Bio Safety Level or pathogen protection level is set of biocontainment precautions designed to isolate dangerous biological agents in a contained biological laboratory. Issued by the US Center of Disease Control or CDC.
- Nonviable testing (particle count). Laser particle counters measure particle density without regard to if they are inert or alive.
- Viable testing (settling plates and growth cultures). Culture plates and tapes to measure density of live organisms such as bacteria, yeast, mold, fungus, virus, etc.
FAQs About Cleanroom Classifications
Q: What Is a Cleanroom?
A: A cleanroom (or clean room) is a room that has HEPA filtration to remove particles from the air. Cleanrooms are used for manufacturing where high levels of cleanliness and sterility are required. Common applications are medical devices, pharmaceutical and semiconductor manufacturing. The FDA mandates the use of cleanrooms to create GMP manufacturing factories.
Q: What Are Cleanrooms Used For?
A: Cleanrooms are used for manufacturing where high levels of cleanliness and sterility are required. Common applications are medical devices, pharmaceutical and semiconductor manufacturing. Cleanrooms use HEPA filters to remove particles from the air. It is common to see pharmaceutical cleanrooms, medical device cleanrooms, semiconductor cleanrooms, electronic cleanrooms, aerospace cleanrooms, food cleanrooms, USP797 compounding pharmacy cleanrooms and biotech cleanrooms. Cleanrooms are also used by the government such as national labs, defense industries, and R&D labs at universities.
Q: How Clean Is a Cleanroom?
A: Very clean. A class 100 cleanroom has 100 particles per cubic foot. By comparison your typical office space has between 500,000 and 1 million particles per cubic foot. Cleanrooms come in different classes from class 100 to 100,000.
Q: When Is a Cleanroom Required?
A: Medical device and pharmaceutical manufacturing requires sterile environments to produce their products. Cleanrooms provide this sterile super clean manufacturing space which reduces the chance of contamination getting in your medicine. Semiconductor manufacturers produce devices with ultra small super dense features. Examples are computer chips for your cell phone or PC. If contamination were to get on the chip during manufacturing, they would not work.
Q: What Does ISO Stand For?
A: ISO is the International Standards Organization. It has created the ISO 4644-1 Cleanroom Standards that describe the allowed number of particles, the allowed size of particles and HEPA filtered air flow changes per hour meet ISO-4, ISO-5, ISO-6, ISO-7, and ISO-8 standard. It relies on measurements per cubic meter. It corresponds to the USA based Fed Standard 209E which relies on measurements per cubic foot. Fed Standard 209E corresponding classes are 10, 100, 1000, 10k and 100k.
Q: What is Clean Room in Pharma?
A: In pharma a clean room is a controlled environment using HEPA filtration to minimize particulate contamination. Pharmaceutical manufacturers are subject to FDA validation of their manufacturing which typically specify use of a clean room to ensure the quality of the manufactured pharmaceutical product. Sterility is highest priority. Pharma cleanrooms focus on both non viable (inanimate) and viable (live) contamination. They typically use laser particle counters to measure non viable contamination levels and settling plates with culture media to measure viable contamination levels. Pharmaceutical cleanrooms use aggressive chemical and UV light cleaning techniques to maintain sterility.
Q: What is a Data Cleanroom?
A: A data cleanroom is a secure isolation virtual platform that typically stores anonymized marketing data from multiple sources. It is used to protect privacy and share data from multiple sources. It is very different from physical cleanrooms used for manufacturing.
Q: How Do Cleanrooms Work?
A: Cleanrooms rely on HEPA or ULPA filtration to remove particles from the air and create an ultra clean environment. With sufficient air changes per hour and laminar air flow it is possible to reduce particulate count from greater then 500k/ft3in typical office space to as low as 100/ft3(class 100 cleanroom). Airlocks are used to prevent contamination from entering the cleanroom. Workers inside cleanrooms typically wear cleanroom garments such as booties and bunny suits to prevent them from bringing contamination into the room. Eating and drinking are never allowed in cleanrooms.
Q: Who Needs a Cleanroom?
A: Industries such as pharmaceutical, medical device and USP797 compounding pharmacies are required by the government to manufacture in sterile environment and must use cleanrooms. Other industries such as semiconductor, electronics, aerospace and optics find the ultra-clean environments in cleanrooms are the only way to cost effectively manufacture their products. Other industries that use cleanrooms include food, beverage, e-liquid, CBD and vitamins.
Q: What is a Pharmaceutical Cleanroom?
A: Pharmaceutical manufacturers are subject to FDA validation of their manufacturing which typically specify use of a clean room to ensure the quality of the manufactured pharmaceutical product. Sterility is highest priority. Pharmaceutical cleanrooms focus on both non viable (inanimate) and viable (live) contamination. They typically use laser particle counters to measure non viable contamination levels and settling plates with culture media to measure viable contamination levels. Pharmaceutical cleanrooms use aggressive chemical and UV light cleaning techniques to maintain sterility.
Q: What is a Class 1 Cleanroom?
A: A class 1 cleanroom refers to ISO standard allowing less than 2 particles greater than 0.3 microns and no particles greater than 1.0 microns per cubic meter. A class 1 cleanroom typically has from 500-750 air changes per hour and typically utilizes ULPA filtration. Other common characteristics are 100% ULPA ceiling coverage and raised floors. It is the cleanest classification.
Q: What is a Class 2 Cleanroom?
A: A class 2 cleanroom refers to ISO standard allowing less than 11 particles greater than 0.3 microns and no particles greater than 1.0 microns per cubic meter. A class 2 cleanroom typically has 500-750 air changes per hour and typically utilizes ULPA filtration. Other common characteristics are 100% ULPA ceiling coverage and raised floors. It is the 2nd most clean classification.
Q: How Many Types of Cleanrooms Are There?
A: The most common type cleanrooms are modular cleanrooms, stick-built cleanrooms (or standard), and softwall cleanrooms. Modular cleanrooms utilize prefabricated modular wall systems which allow for faster installation, easy modification(s) and reasonable cost. Stick built or standard cleanrooms rely on conventional steel stud(s) drywall construction. It can be slightly cheaper. Softwall cleanrooms use clear vinyl curtains suspended from metal frames. Small softwall cleanrooms are often mounted on casters so they can be easily moved around.
Q: What is a Class 4 Cleanroom?
A: A class 4 cleanroom refers to ISO standard allowing less than 1020 particles greater than 0.3 microns and less than 2 particles greater than 5.0 microns per cubic meter. A class 5 cleanroom requires from 500-600 air changes per hour and typically utilizes ULPA filtration. Other common characteristics are 100% ULPA ceiling coverage and raised floors. It is the 4th most clean classification.
Q: How Do You Prepare For Cleanroom / Installations?
A: If you need an ISO-7 cleanroom you should prepare your facility for the modular cleanroom installation. Check that area is free and clear up to height of cleanroom. Frequently existing electrical conduit, lighting, sprinklers, and HVAC ducting need to be relocated. When installing new cleanroom flooring make sure the existing concrete is in good shape. Have any cracks and depressions filled to level the floor.
What Are the Do and Don’ts in a Cleanroom?
Do:
- Do wipe down all surfaces on a regular basis to remove contamination.
- Do make sure doors are always closed to maintain positive pressure.
- Do always have all staff were cleanroom suits over their street clothes to prevent bringing contamination into the cleanroom.
Don't:
- Don’t eat or drink in your cleanroom.
- Don’t bring dirty equipment or material into cleanroom – always wipe it down before bringing it in.
- Don’t turn off the HEPA fan filter units – it will take several hours after you turn them back on before the cleanroom is clean again.
ISO Cleanroom Specifications
The ISO 1 specification for cleanrooms require less than 2 particles greater than 0.3 microns and no particles greater than 1.0 microns per cubic meter. An ISO 1 cleanroom typically has from 500-750 air changes per hour and typically utilizes ULPA filtration. Other common characteristics are 100% ULPA ceiling coverage and raised floors.It is the most clean of the cleanroom classification.
The ISO 2 specification for cleanrooms requires less than 11 particles greater than 0.3 microns and no particles greater than 1.0 microns per cubic meter. A IS0 2 cleanroom typically has 500-750 air changes per hour and typically utilizes ULPA filtration. Other common characteristics are 100% ULPA ceiling coverage and raised floors.It is the 2ndmost clean classification.
The ISO 3 specification for cleanrooms requires less than 102 particles greater than 0.3 microns and no more than 8 particles greater than 1.0 microns per cubic meter. A IS0 3 cleanroom typically has 500-750 air changes per hour and typically utilizes ULPA filtration. Other common characteristics are 100% ULPA ceiling coverage and raised floors.It is the 3rd most clean classification.
The ISO 4 specification for cleanrooms requires less than 1020 particles greater than 0.3 micronsand no more than 2 particles greater than 5.0 microns per cubic meter. An IS0 4 cleanroom typically has 500-600 air changes per hour and typically utilizes ULPA filtration. Other common characteristics are 100% ULPA ceiling coverage and raised floors.It is the 4th most clean classification.
The ISO 5 is a super clean cleanroom classification. A cleanroom must have less than 3,520 particles >0.5 micron per cubic meter and 250-300 HEPA filtered air changes per hour. The equivalent FED standard is class 100 or 100 particles per cubic foot. Common applications are semiconductor manufacturing and pharmaceutical filling rooms.
The ISO 6 is a very clean cleanroom classification. A cleanroom must have less than 35,200 particles >0.5 micron per cubic meter and 180 HEPA filtered air changes per hour. The equivalent FED standard is class 1000 or 1000 particles per cubic foot.
The ISO 7 is a common clean cleanroom classification. A cleanroom must have less than 352,000 particles >0.5 micron per cubic meter and 60HEPA filtered air changes per hour. The equivalent FED standard is class 10.000 or 10,000 particles per cubic foot. Common applications are pharmacy USP800 compounding rooms, electronics manufacturing and medical device manufacturing.
The ISO 8 is the least clean cleanroom classification. A cleanroom must have less than 35, 200,000 particles >0.5 micron per cubic meter and 20 HEPA filtered air changes per hour. By comparison a typical office space would be 5-10 times more dirty. The equivalent FED standard is class 100,000 or 100,000 particles per cubic foot. Common applications include plastic extrusion for medical devices, e-liquid manufacturing, and nutraceutical packaging.
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